FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365360
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1604.8861693E-835.998755
CGAGTAG16550.034.3673863
CCGAGTA16100.033.99782
CGGAGGT19950.033.562747
GCTGGGA127250.032.476679
TTGGGAG105350.032.3937456
GGAGGCT108350.032.028429
AGTAGCT68350.031.2849395
GAGTAGC42350.031.2810844
GGGAGGC122600.031.0070788
GGAGGTT55050.030.4730538
TACTCGG17500.030.4446543
CTACTCG17600.029.872622
CGCGACT1952.7381793E-729.582371
GTAGCTG73200.029.0153836
CTCGGGA19900.028.5819195
TGGGAGG129700.028.3660137
AGGCGGA26900.027.5678124
TAGCTGG77100.027.3609147
GGCGGAG28650.027.1404175
Produced by FastQC (version 0.11.5)