FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365360
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17100.039.9997062
TTGGGAG104600.039.6478966
TACTCGG17850.039.1253973
CGAGTAG17150.039.043083
CGGAGGT23950.038.4798857
CTACTCG18300.037.3767742
GCTGGGA136650.036.8826189
AGTAGCT70600.036.20375
GAGTAGC44650.035.959094
GGGAGGC135850.034.1845138
GGAGGCT116950.034.1685189
CCCGAGT25000.033.696381
GTAGCTG74700.033.638316
GGAGGTT61650.033.1676458
CTCGGGA21750.033.1032075
GCTACTC46400.032.7417531
AGGCGGA33000.032.7267274
TAGCTGG79900.032.260097
GGCGGAG36250.032.1763155
TCGGGAG21550.031.7399066
Produced by FastQC (version 0.11.5)