FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867124
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17850.044.374633
CCGAGTA18200.044.3216742
GCTGGGA142500.039.9653789
AGTAGCT74700.039.9026765
TTGGGAG112150.038.5189326
CGGAGGT22250.038.1833727
CCCGAGT25700.037.8936961
GAGTAGC49050.037.7285354
GTAGCTG76800.037.499126
GGGAGGC138050.035.673198
TACTCGG21550.035.419213
CTCGGGA21550.035.414485
TAGCTGG90500.035.1638157
GGAGGTT62250.034.8136378
GGAGGCT113700.034.3210959
TCGCGTT1905.817128E-934.1044585
TCGGGAG24500.033.501256
TGGGAGG145900.033.3097047
CTACTCG21250.032.8762552
GCTACTC48950.032.1269951
Produced by FastQC (version 0.11.5)