FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867124
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19650.049.830422
CGAGTAG19300.049.614953
GAGTAGC44200.047.2382624
CCCGAGT23500.045.0432971
AGTAGCT74450.044.8715935
TTGGGAG116250.042.7960136
GCTGGGA148600.042.539659
TACTCGG19950.041.1415063
GTAGCTG78150.040.8124776
CTACTCG19900.040.521282
GGAGGCT122300.039.0894749
GGGAGGC150050.038.577878
TAGCTGG86200.038.1704377
TCGCGTT1752.6047928E-937.0273555
CGGAGGT26200.035.7240147
GCTACTC53850.035.1682431
GCACTTT109850.035.135441
TGGGAGG158200.034.679017
CTCGGGA25650.033.68315
TTTGGGA123950.033.3413775
Produced by FastQC (version 0.11.5)