FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004149
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13000.058.173272
AGTAGCT50400.056.9981655
GAGTAGC31600.056.7323464
CGAGTAG12750.055.904083
GTAGCTG56150.052.699996
TTGGGAG83150.050.134286
GCTGGGA112800.048.764389
TAGCTGG62100.048.2303287
CCCGAGT17950.047.415181
CTACTCG14200.045.1418342
ATTCGCG803.8930553E-544.998554
GCTACTC38050.043.4090921
TACTCGG14450.043.348083
GGAGGCT90000.043.2786069
GGGAGGC116450.042.7225388
GGAGGTT45900.041.5672878
TTTGGGA86150.040.699795
TGGGAGG115500.039.6454737
CGGAGGT20050.039.4999737
GCACTTT74050.037.987391
Produced by FastQC (version 0.11.5)