FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004149
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13000.062.58423
CCGAGTA13700.060.4375572
TACTCGG17450.058.589873
CTACTCG16950.056.919982
GAGTAGC32350.056.0861854
AGTAGCT50400.055.7139175
TTGGGAG87900.055.617386
CTCGGGA19900.050.6529355
GCTGGGA120950.050.599099
CCCGAGT19400.049.3629651
GCTACTC39000.049.109821
GTAGCTG59100.048.2433366
GGAGGCT101000.047.3343439
GCACTTT76400.046.6512371
TAGCTGG64550.046.6240167
TCGGGAG22350.046.3889546
GGGAGGC129400.045.681318
TTTGGGA88050.045.0559235
CACTTTG80200.044.1692852
ACTTTGG79050.043.7188763
Produced by FastQC (version 0.11.5)