FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117435
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20650.059.6207543
CCGAGTA21700.057.7312432
AGTAGCT83450.052.4569365
GAGTAGC50800.051.447514
GTAGCTG88350.048.6511886
GCTGGGA174350.048.3984389
TTGGGAG134250.047.623836
TAGCTGG100350.045.3445787
CCCGAGT28950.045.0157131
GGAGGCT148900.041.9711349
TACTCGG24350.041.690933
CGGAGGT29600.041.5939457
GGGAGGC183600.041.332688
GCACTTT117100.040.5808561
GGAGGTT75000.039.839378
CTACTCG25000.039.4549942
TCGGGAG31050.038.724036
TGGGAGG183800.038.2714357
TTTGGGA142800.037.4615975
GCTACTC59150.037.126091
Produced by FastQC (version 0.11.5)