FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5406341
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20450.049.3104442
CGAGTAG20000.048.9580653
GAGTAGC48450.044.283084
AGTAGCT82100.043.4964525
CGGAGGT25400.041.384197
TTGGGAG123600.040.3673366
GTAGCTG87700.039.8980836
GCTGGGA162250.039.40439
CTACTCG21550.038.437462
CCCGAGT28000.038.380221
TAGCTGG95200.037.889267
TACTCGG21100.037.875283
GGGAGGC158650.036.4410488
GGAGGCT134800.035.1440129
GGAGGTT72150.034.626498
TGGGAGG164350.033.5125167
GCTACTC53900.032.783591
CTCGGGA27000.031.9987375
TCGGGAG26700.031.8189686
GAGGTTG92550.031.5838419
Produced by FastQC (version 0.11.5)