FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5406341
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19750.052.8600432
CGAGTAG19500.051.691613
AGTAGCT81200.048.4135865
GAGTAGC49150.046.143834
GCTGGGA173500.045.898069
TTGGGAG129600.045.4442526
GTAGCTG89800.043.0554966
CCCGAGT26450.042.465241
CGGAGGT28100.042.0211757
GGAGGCT136500.040.98529
TACTCGG23450.040.835273
CTACTCG23450.040.5282362
TAGCTGG99350.040.0763247
GGGAGGC169200.039.8296178
GCTACTC56100.037.989491
TGGGAGG170650.036.2846377
GGAGGTT75500.035.9521648
CTCGGGA25550.035.7884945
TCGGGAG27650.034.8931586
GCACTTT120000.034.8601721
Produced by FastQC (version 0.11.5)