FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390256
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21450.057.7338942
CGAGTAG20500.057.2478873
AGTAGCT84300.051.501455
GAGTAGC51050.051.4781344
CCCGAGT27650.048.694541
GTAGCTG88000.047.6178786
GCTGGGA166200.046.3972439
TTGGGAG133850.044.5391246
TAGCTGG99900.043.387117
TACTCGG21550.040.0921823
GGGAGGC169250.039.2647448
GGAGGCT138650.038.5316669
CGGAGGT30150.037.014697
CTACTCG22900.036.1569752
TGGGAGG174200.036.082437
GCTACTC55600.035.8706051
GGAGGTT77150.035.556488
TTTGGGA143750.034.3093455
GCACTTT121850.034.2717481
GTGCTGG116500.033.187777
Produced by FastQC (version 0.11.5)