FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6587828
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22600.035.6926272
CGAGTAG21050.035.2286343
GCTGGGA162950.034.2860959
TACTCGG23550.033.93463
CTACTCG23350.033.620842
AGTAGCT85400.032.625995
TAGCTGG93800.031.6231447
GAGTAGC54600.031.1193144
CGGAGGT27600.030.5202057
GGAGGTT70000.030.3413338
TCGGGAG30950.030.2408076
GTAGCTG92750.029.9629146
GGAGGCT140000.029.9299269
TTGGGAG127400.029.7818626
GGGAGGC168650.028.6875578
CCCGAGT32450.028.4566421
GCTACTC58850.028.072261
CTCGGGA30000.026.3986725
TGGGAGG170150.025.938167
AGGCGGA37450.025.1843174
Produced by FastQC (version 0.11.5)