FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6587828
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21900.042.4103783
CCGAGTA22600.041.7342572
GCTGGGA175650.036.237129
AGTAGCT88450.035.8988465
TTGGGAG134750.034.838436
GAGTAGC55300.034.6324354
TAGCTGG101700.032.708517
CCCGAGT32950.032.5582241
GTAGCTG96050.032.3836756
GGAGGCT150750.032.3833669
TACTCGG24300.031.703273
GGGAGGC176900.030.6890378
CGGAGGT29650.030.3546357
CTACTCG24850.030.1326142
TGGGAGG180700.029.087377
GGAGGTT77850.027.8386148
TCGGGAG31050.026.4352256
GTGCTGG123950.025.6172087
AGGCGGA38300.025.566234
CCCAAAG159750.024.698441
Produced by FastQC (version 0.11.5)