FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4494935
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26100.067.308813
AGTAGCT96150.067.24345
GAGTAGC61800.064.658724
CCGAGTA27450.064.2744142
GTAGCTG103850.061.148346
TTGGGAG154950.056.9667556
GCTGGGA207500.056.2454649
CCCGAGT34650.052.8615151
TAGCTGG122450.052.741967
GCACTTT128450.049.2350771
GGAGGCT172750.048.512939
TTTGGGA148100.048.4688535
AAGTGCT121650.046.578475
CAAGTAG46700.046.5599673
ACTTTGG135550.046.104453
CCAAGTA49150.045.8601232
AAGTAGC48600.045.6285934
CACTTTG138700.044.8072972
GGGAGGC231450.044.7637258
CTACTCG31150.043.9248162
Produced by FastQC (version 0.11.5)