FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4494935
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26800.073.342923
CCGAGTA28350.070.094892
AGTAGCT101600.070.0161745
GAGTAGC64500.068.98574
GTAGCTG108650.065.2742166
TTGGGAG150950.061.0537036
GCTGGGA220800.060.5547189
TAGCTGG127050.056.7276157
CCCGAGT37350.053.9774321
GGAGGCT174000.053.421029
TACTCGG31400.052.7385863
CTACTCG30900.052.193912
GCACTTT123250.051.81811
TTTGGGA145350.051.3687325
AAGTGCT129250.051.1941265
GCTACTC73350.050.6517451
CAAGTAG45700.050.1004073
GGGAGGC231050.049.2675638
TGAGTAG51000.047.7174033
ACTTTGG134200.047.6957363
Produced by FastQC (version 0.11.5)