FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4935746
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18950.045.211893
GCTGGGA147500.044.9554679
AGTAGCT75900.043.9191675
GAGTAGC49150.043.21294
CCGAGTA20300.042.9336552
GTAGCTG78450.042.124496
CGGAGGT23350.041.625727
TAGCTGG87400.040.4468777
TTGGGAG107500.039.71566
TACTCGG19050.038.9275553
CTACTCG18600.038.3381162
GGAGGCT118900.037.663749
CCCGAGT25500.036.487851
GGGAGGC138750.035.4407278
GCTACTC49550.034.4987561
GGAGGTT62850.034.137048
TCGGGAG23500.032.781656
GTGCTGG105300.031.9987037
TGGGAGG142400.031.7515227
AAGTGCT109250.031.6984865
Produced by FastQC (version 0.11.5)