FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4935746
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17650.051.398913
CCGAGTA18000.050.799482
TTGGGAG116950.047.7742586
GAGTAGC45650.045.5811354
AGTAGCT76500.043.8588185
TACTCGG22150.043.2320673
GCTGGGA149650.043.1085859
GGAGGCT124950.042.986799
CGGAGGT27900.042.5806437
GTAGCTG78750.041.417146
CTACTCG21650.041.2374532
GGGAGGC154750.041.1760868
CCCGAGT24650.040.0166321
TAGCTGG87200.037.4862337
GGAGGTT72600.037.0909088
TGGGAGG158400.037.0454527
CTCGGGA24050.036.8232845
TTTGGGA126950.036.4678965
GCTACTC54700.036.4610631
ACTTTGG114600.034.6174473
Produced by FastQC (version 0.11.5)