FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934220
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23950.062.5276153
AGTAGCT89850.059.0559165
CCGAGTA25450.058.298362
GAGTAGC59200.057.038124
GTAGCTG96900.054.313486
GCTGGGA190800.050.7148259
CCCGAGT32800.048.8082771
TAGCTGG111000.047.2844777
TTGGGAG137850.046.4833376
CTACTCG29000.043.9594422
TACTCGG29250.043.0750923
GCTACTC63550.042.439511
GGAGGCT152600.042.0847139
GGGAGGC191500.039.5137258
TTTGGGA139300.038.608555
TCGGGAG36150.038.239046
AAGTGCT121000.037.7240145
GCACTTT122150.037.48821
CGGAGGT31500.037.2555587
CTCGGGA34400.037.0449375
Produced by FastQC (version 0.11.5)