FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934220
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25750.067.386023
CCGAGTA27250.063.4124762
GAGTAGC56350.061.84174
AGTAGCT89300.060.2287035
GTAGCTG95800.055.315486
TTGGGAG151500.054.748756
CCCGAGT34050.052.8636631
GCTGGGA197150.051.714189
TAGCTGG112800.049.212987
GGAGGCT162250.046.374029
TTTGGGA147650.045.496985
GCACTTT130600.045.0966721
CTACTCG28450.044.5410422
GGGAGGC213700.044.305298
TACTCGG29150.044.2124373
GCTACTC66800.044.1918031
TCGGGAG33650.044.0774546
CTCGGGA32150.042.998635
ACTTTGG136050.042.866343
CGGAGGT33900.042.4780547
Produced by FastQC (version 0.11.5)