FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256229
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG114050.042.2957956
AGTAGCT78400.042.1516275
TACTCGG20550.039.9402473
CGAGTAG19700.039.836213
GCTGGGA157850.039.2713939
GAGTAGC50850.039.0783124
CTACTCG20800.038.432372
GTAGCTG84000.038.3986936
CCGAGTA21200.038.3866422
GGAGGCT128650.037.7196669
TAGCTGG93100.036.037447
GGGAGGC157550.035.96458
CGGAGGT25800.035.4406557
CTCGGGA27150.034.4739655
TCGGGAG28300.034.0907146
GGAGGTT66450.034.0214168
GCTACTC53500.033.4244841
TGGGAGG158400.032.6807067
CCCGAGT27650.032.3365251
TTTGGGA129750.030.4637035
Produced by FastQC (version 0.11.5)