FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5413745
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21000.050.0570142
TTGGGAG126100.046.7056666
AGTAGCT78900.046.53985
GAGTAGC51450.046.1806374
CGAGTAG21150.045.2764783
TACTCGG22850.043.4834753
GCTGGGA157450.042.025829
CTACTCG22450.041.6925432
GTAGCTG85950.041.549636
CCCGAGT28400.041.3234521
GGAGGCT138350.040.2294169
GGGAGGC166650.040.136718
CGGAGGT27300.039.5603337
TAGCTGG97750.037.7124337
TGGGAGG170050.037.344217
GCTACTC55950.037.0612261
TCGGGAG29600.036.2431456
GCACTTT119200.036.0599751
CTCGGGA28300.035.3638655
TTTGGGA134950.034.519365
Produced by FastQC (version 0.11.5)