FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3926173
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT98700.084.1061255
CGAGTAG28900.077.727063
GAGTAGC65150.077.135894
GTAGCTG107750.075.705626
CCGAGTA31350.073.5180362
TTGGGAG151550.065.037376
TAGCTGG129800.064.952777
GCTGGGA231800.064.045819
AAGTAGC46300.061.5785834
CAAGTAG45000.059.6776663
GCACTTT122000.059.5333251
AAGTGCT115450.059.4935535
CCAAGTA47900.058.3415032
TTTGGGA138150.058.3169335
CCCGAGT40850.057.5593721
ACTTTGG127350.055.9133
TGAGTAG47100.055.1825523
GGAGGCT172600.054.769589
CACTTTG131200.054.567492
CTGAGTA48900.054.3499222
Produced by FastQC (version 0.11.5)