FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6272356
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27200.035.7402533
CCGAGTA29050.033.2202872
GAGTAGC62900.031.253694
AGTAGCT100100.030.2810925
GTAGCTG108850.028.0453536
GCTGGGA203300.027.127829
TAGCTGG120550.026.3387327
CCCGAGT41800.025.7159941
TACTCGG26300.024.6422023
CTACTCG26150.023.4094872
GCTACTC64850.021.6929171
TTGGGAG139150.021.628016
GGAGGCT159050.019.7367829
CCCAAAG164900.019.2497621
AAGTGCT128550.019.2108025
GTGCTGG134200.018.8848557
CCAAAGT148100.018.7705482
AGCTGGG181400.018.7339028
TCGGGAG37800.018.666286
GGGAGGC188750.018.6147148
Produced by FastQC (version 0.11.5)