FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6533787
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG29750.054.978313
CCGAGTA33550.051.5431752
AGTAGCT116650.050.245
GAGTAGC77600.047.789814
GTAGCTG123150.047.062126
CGGAGGT38750.045.520227
GCTGGGA240900.045.1881989
TTGGGAG176700.044.4119646
CCCGAGT45250.042.0083351
TAGCTGG147150.041.1477177
GGAGGCT186000.040.4882289
GGAGGTT96000.037.9480368
GGGAGGC243050.037.1459928
TCGCGTT2401.8189894E-1235.9981355
TTTGGGA180850.035.948375
GCACTTT157950.035.6464351
AGGCGGA57350.035.4083334
TACTCGG35500.035.1131863
TGGGAGG242850.034.626997
CTACTCG34650.034.103042
Produced by FastQC (version 0.11.5)