FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4277677
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG31750.076.2320563
AGTAGCT116200.076.024235
CCGAGTA33300.075.7122042
GTAGCTG122700.070.529946
GAGTAGC75700.069.721614
TTGGGAG174500.061.5583536
GCTGGGA252450.060.5178649
TAGCTGG149650.060.330157
CCCGAGT43200.059.5367431
CCAAGTA52550.058.943762
CAAGTAG51450.058.1038363
TTTGGGA156850.057.2394645
GCACTTT140450.056.634931
AAGTAGC55100.055.538824
ACTTTGG148200.053.272673
CACTTTG150750.051.8936922
AAGTGCT122900.050.614565
CTGAGTA56350.048.960632
GGAGGCT194500.048.824619
TGAGTAG55200.048.1536673
Produced by FastQC (version 0.11.5)