FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391421
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25950.044.696353
CCGAGTA28000.044.254492
AGTAGCT101950.044.137515
GTAGCTG108750.041.0466236
GCTGGGA200700.040.8952149
TTGGGAG158250.040.4457666
GAGTAGC67700.040.0988854
CGGAGGT30300.038.7311177
CCCGAGT35800.037.5352251
GGGAGGC194950.036.7464078
TAGCTGG125900.036.427457
GGAGGCT154950.034.337449
TGGGAGG205950.034.119537
GGAGGTT77450.033.5583658
AGGCGGA44400.031.6250514
GCACTTT151400.031.5416721
TTTGGGA168500.031.319815
CTACTCG29250.030.2946662
GGAGGCC119800.029.7483849
GTGCTGG129750.029.6866667
Produced by FastQC (version 0.11.5)