FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391421
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28900.057.2985463
CCGAGTA30750.055.2691042
GAGTAGC66500.050.3436744
CCCGAGT40900.047.242221
AGTAGCT106550.046.8266835
GTAGCTG109800.045.4406476
GCTGGGA206400.044.6143389
TTGGGAG154650.043.8079876
CTACTCG27150.042.173812
TAGCTGG126050.040.839167
GGAGGCT159400.040.831359
TACTCGG29300.038.824253
GGGAGGC204200.038.3607258
CGGAGGT31850.037.750327
GCTACTC70550.037.709271
CTCGGGA31400.036.9155965
TCGGGAG34150.036.051146
TGGGAGG205150.035.3755047
GGAGGTT79750.034.7571078
TTTGGGA162500.033.6280755
Produced by FastQC (version 0.11.5)