FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5425238
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18900.047.2373053
CCGAGTA20600.045.7856452
GAGTAGC48450.040.2717744
GCTGGGA152200.039.6419149
AGTAGCT78600.039.5718575
TTGGGAG118250.039.2111856
GTAGCTG84500.036.6384436
CTCGGGA23650.036.5321585
TACTCGG20950.036.0853163
GGGAGGC152150.035.9165888
GGAGGCT131250.035.6016929
CCCGAGT29400.035.2679631
TAGCTGG94500.034.666097
TCGGGAG27650.034.1115466
CTACTCG21350.033.7230872
TGGGAGG155850.032.476847
GCTACTC52550.032.337361
CGGAGGT23900.031.932527
TCGCGTT2507.6397555E-1131.679475
AGGCGGA34300.030.436814
Produced by FastQC (version 0.11.5)