FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162317
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23150.055.3597953
CCGAGTA24400.052.228662
AGTAGCT85600.049.2889185
GAGTAGC56050.048.042044
GTAGCTG93000.045.986356
GCTGGGA180000.044.5592779
CGGAGGT27700.044.4469347
TTGGGAG133800.044.286286
TAGCTGG103950.042.80457
CCCGAGT30550.041.2507861
TACTCGG22900.040.8726963
CTACTCG23050.039.9820022
GGAGGCT140100.039.468319
GGGAGGC179000.038.2518968
GGAGGTT73600.038.1515548
GCACTTT122950.036.5478171
TTTGGGA141450.036.3429955
TGGGAGG181050.036.0690087
TCGGGAG31450.034.7975276
CTCGGGA29700.034.1812675
Produced by FastQC (version 0.11.5)