FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5423252
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG34000.079.834353
AGTAGCT125400.076.592395
GAGTAGC81050.074.530884
CCGAGTA37300.073.5433652
GTAGCTG137800.068.707466
GCTGGGA287300.061.7994779
TTGGGAG198200.060.4836466
TAGCTGG162400.060.250527
CCCGAGT48300.058.445951
GCACTTT159450.056.003171
CAAGTAG59000.053.6942753
ACTTTGG166600.053.459153
AAGTGCT156800.052.9891665
TTTGGGA189100.052.8856775
CACTTTG169750.052.0005652
CCAAGTA62800.051.935692
GGAGGCT218150.050.430769
AAGTAGC65600.049.2799034
CTACTCG41150.048.990912
TGAGTAG65150.048.7361833
Produced by FastQC (version 0.11.5)