FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178019
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23850.057.0626373
CCGAGTA26700.051.7877582
AGTAGCT92400.051.1937875
GAGTAGC55350.049.5652584
GTAGCTG99850.047.878876
GCTGGGA200000.044.855119
TTGGGAG142150.044.72376
TAGCTGG113150.044.1599737
CCCGAGT35150.043.3015821
CTACTCG26800.040.0394942
GGAGGCT158150.039.9713869
GGGAGGC194000.037.6693538
TACTCGG29600.036.7336123
TCGGGAG34200.036.420336
GCACTTT118900.035.309191
AAGTGCT124800.035.306995
TTTGGGA142400.035.1903155
TGGGAGG196400.034.4596027
GCTACTC66300.034.054731
CAAGTAG49650.033.211973
Produced by FastQC (version 0.11.5)