FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178019
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23750.058.5091633
AGTAGCT89650.054.1296965
GAGTAGC58450.054.0761764
CCGAGTA26450.053.6254272
GTAGCTG98250.049.0252236
CTACTCG30700.046.670762
TACTCGG32400.046.44423
GCTGGGA195550.046.0601659
CCCGAGT33750.045.657061
GCTACTC65300.045.4309651
TTGGGAG138850.045.009056
TAGCTGG109500.044.7774167
GGAGGCT160950.042.18399
CTCGGGA36500.040.0432365
TCGGGAG38550.038.287366
ACTCGGG40200.037.7904784
GGGAGGC204050.037.71968
GCACTTT122250.036.989521
TTTGGGA144250.035.986985
AAGTGCT123300.035.7366945
Produced by FastQC (version 0.11.5)