FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5720266
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19350.036.4712523
CCGAGTA20400.036.011412
TACTCGG19850.034.4642373
AGTAGCT75700.033.0982745
GCTGGGA139550.032.916449
CTACTCG19350.032.382352
GAGTAGC50500.031.228474
CGGAGGT22300.030.9947687
GTAGCTG81450.030.4081066
CCCGAGT29550.030.0249821
CTCGGGA22950.029.8032045
GGAGGCT119350.029.6800569
TTGGGAG118200.029.6642596
TAGCTGG88900.028.912697
GGGAGGC145500.028.5024058
TCGCGTT1555.771606E-527.8702965
TCGGGAG26300.027.3757676
GGAGGTT58000.026.689018
GCTACTC52250.026.230251
TGGGAGG147850.026.1015017
Produced by FastQC (version 0.11.5)