FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252812
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18000.047.1989063
CCGAGTA19550.045.666462
TACTCGG20550.045.1960333
CTACTCG20500.043.1989972
TTGGGAG112450.042.3218276
GCTGGGA144700.040.999749
GAGTAGC49400.040.8087734
AGTAGCT77400.040.4641765
CGGAGGT23950.039.9823727
GGAGGCT120300.038.6624459
GTAGCTG81300.038.2574126
GGGAGGC147400.037.6599128
CCCGAGT26800.037.0781
TAGCTGG89600.036.3205837
CTCGGGA25350.035.5021365
TGGGAGG149400.034.8907557
TCGGGAG27100.034.5379456
GCTACTC51050.034.1343541
GGAGGTT63900.033.3513378
TCGCGTT1957.496055E-933.2299965
Produced by FastQC (version 0.11.5)