FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022533
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23050.049.056022
CGAGTAG22550.047.5799453
GAGTAGC52650.043.9019364
AGTAGCT87800.043.379455
CCCGAGT33400.040.3876081
CGGAGGT27900.039.999127
GCTGGGA171700.039.0393269
GTAGCTG96100.038.808726
TTGGGAG129900.037.412576
TAGCTGG109350.035.29147
CTACTCG23700.034.6433682
GGGAGGC163300.034.3017548
GGAGGCT139600.034.2972379
CTCGGGA26150.034.140745
TACTCGG24600.033.9552653
TCGCGTT2800.033.4278375
TCGGGAG29600.032.5938766
GGAGGTT74800.032.0527738
AGGCGGA40250.031.1286544
GCGGAGG37600.030.8291076
Produced by FastQC (version 0.11.5)