FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5241225
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG30500.068.2313843
CCGAGTA32650.064.408592
AGTAGCT113300.060.179455
GAGTAGC72550.059.650614
GTAGCTG119950.057.743486
CCCGAGT42350.052.2823831
GCTGGGA251200.052.222419
TAGCTGG139650.051.1960537
TTGGGAG168500.050.1644866
GGAGGCT191050.045.600179
TACTCGG34300.044.2970163
CTACTCG34350.044.238452
TTTGGGA157550.043.048765
GCTACTC78600.042.0255431
GCACTTT141400.041.7738881
AAGTGCT139950.041.6716085
CCAAGTA58950.040.6822132
CAAGTAG54750.040.5091133
ACTTTGG147100.040.3367353
TCGGGAG41900.039.1786046
Produced by FastQC (version 0.11.5)