FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5241225
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28750.068.867573
CCGAGTA30800.066.387672
AGTAGCT107900.065.19185
GAGTAGC69250.063.9403844
GTAGCTG120350.058.986246
TTGGGAG177900.053.138426
GCTGGGA258600.052.1191379
TAGCTGG145450.051.232617
CCCGAGT41550.050.9516561
TACTCGG35900.049.7367133
CTACTCG35650.049.2776682
GCACTTT138800.048.818251
GGAGGCT200350.047.4715429
TTTGGGA165500.045.8089375
ACTTTGG146750.045.774483
CACTTTG148200.044.9865462
TCGGGAG43650.044.6997346
AAGTGCT143050.044.2909165
GGGAGGC258850.044.22518
GCTACTC82750.043.6835331
Produced by FastQC (version 0.11.5)