FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5309222
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24500.056.4409522
CGAGTAG24050.054.19733
AGTAGCT90750.049.1093375
GAGTAGC57050.048.0918164
GTAGCTG95650.045.9160926
CCCGAGT32450.045.8034171
TTGGGAG129600.045.2209246
GCTGGGA177500.043.7666329
TAGCTGG107950.042.3517277
CGGAGGT27950.040.1848957
GGAGGCT143150.040.1357739
GGGAGGC176500.038.4260948
CTACTCG25200.038.0110862
TACTCGG25350.037.782253
GGAGGTT70700.035.8462148
TTTGGGA134550.035.3702285
TGGGAGG178250.035.1406487
TCGGGAG27650.034.892316
GCTACTC62750.034.0348131
GCACTTT124500.034.018421
Produced by FastQC (version 0.11.5)