FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5309222
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22800.056.5248373
CCGAGTA23700.054.3783232
AGTAGCT92800.047.1707345
TTGGGAG141000.047.0281146
GCTGGGA187050.046.8820539
GAGTAGC57700.046.4177554
GTAGCTG95700.046.042256
TACTCGG26750.042.525993
CCCGAGT30450.042.32881
TAGCTGG110900.042.133767
CTACTCG26850.041.563162
GGAGGCT152800.041.3231359
GGGAGGC191350.040.9371388
GCTACTC62300.039.2971531
CGGAGGT31900.039.045637
TCGGGAG32500.038.7678536
GCACTTT128800.037.4007641
GGAGGTT75300.037.2895138
TGGGAGG187900.037.129077
TTTGGGA145800.036.8875775
Produced by FastQC (version 0.11.5)