FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178018
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17700.045.558572
CGAGTAG18050.040.2874263
TTGGGAG104950.038.8292856
GGAGGCT105050.037.215599
GGGAGGC131800.036.4907048
GCTGGGA134950.036.1191029
GAGTAGC42800.035.831194
CGGAGGT22400.035.356227
GCTACTC44400.034.704811
CTACTCG18100.034.6071662
CTCGGGA21650.033.920925
AGTAGCT73300.033.8875735
CCCGAGT25500.033.8844151
GGAGGTT61150.033.2027328
TACTCGG18650.032.8144723
TGGGAGG138550.032.790197
TCGGGAG24300.032.5920566
GTAGCTG76350.032.0623446
TAGCTGG81750.031.5294517
AGGCGGA31950.030.6473834
Produced by FastQC (version 0.11.5)