FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5035894
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1701.9608706E-938.1166425
CGAGTAG16850.034.615523
TTGGGAG95350.033.4506156
AGTAGCT64700.033.161415
CCGAGTA17550.032.828122
GCTGGGA116950.032.3822149
GAGTAGC40750.031.099514
GTAGCTG67650.030.970366
AGGCGGA25700.030.8196754
CGGAGGT18050.030.7138737
GGGAGGC116050.030.586018
TACTCGG17450.030.5366943
CGCGTTG2202.4265319E-829.453776
CGGGTTC14050.029.2091969
TAGCTGG74500.029.185817
GGCGGAG27650.029.1637885
GGAGGTT50850.028.4594278
CCCGAGT25700.028.3504281
GGAGGCT103500.028.173179
TAGCGCG1555.7715595E-527.8702345
Produced by FastQC (version 0.11.5)