FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5954404
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20300.036.184143
CCGAGTA21750.034.4389722
TACTCGG19050.032.5100483
CTACTCG18900.032.0105862
GCTGGGA140750.030.947819
AGTAGCT78550.030.705885
GAGTAGC53050.029.9987224
CGGAGGT21100.029.6865487
GTAGCTG83000.029.5800726
TTGGGAG112000.029.5064936
TAGCTGG87600.029.013067
GGGAGGC137900.028.7158288
TCGGGAG26300.028.4708586
GCTACTC50000.027.8496191
GGAGGCT119650.027.74039
CCCGAGT30900.027.3186551
CTCGGGA24250.027.3148635
AGGCGGA28950.026.3665394
GGCGGAG31200.026.0763475
TGGGAGG145100.025.3557457
Produced by FastQC (version 0.11.5)