FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5954404
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17700.037.4228483
CCGAGTA18250.036.2950362
AGTAGCT80050.034.2677735
CGGAGGT22700.034.25477
GCTGGGA151850.033.7588359
TTGGGAG111850.033.3438726
TCGCGTT2603.6379788E-1233.2299845
TACTCGG22650.033.0588233
GAGTAGC48050.032.3655244
GTAGCTG86500.031.0466656
TCGGGAG28500.030.8203266
GGAGGCT130700.030.5731129
GGGAGGC149150.030.459898
CTCGCGT4500.030.3992844
CTACTCG23600.030.2026792
CTCGGGA26500.029.886095
TAGCTGG94200.029.3496277
AGGCGGA33800.029.1827434
GGAGGTT66650.029.0585788
GGCGGAG34800.028.551055
Produced by FastQC (version 0.11.5)