FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137646
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26200.048.6461373
CCGAGTA28400.047.1639182
AGTAGCT98300.041.8221055
GAGTAGC63200.041.0161364
CCCGAGT37750.039.365241
GTAGCTG104400.039.240556
CTACTCG26700.038.8388632
TACTCGG28200.038.812433
GCTGGGA199750.037.2337539
TTGGGAG147200.036.8796586
TAGCTGG116600.036.060997
GGAGGCT155900.035.9299669
CGGAGGT31000.035.534737
TCGGGAG34350.035.4228326
GGGAGGC194250.034.5073788
GCTACTC64900.033.345651
CTCGGGA32900.033.2637375
TGGGAGG197950.030.6252677
GGAGGTT80800.029.761758
CAAGTAG54350.028.0874753
Produced by FastQC (version 0.11.5)