FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137646
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24750.051.490073
CCGAGTA26800.049.7006842
AGTAGCT94150.043.588955
GAGTAGC60000.042.838954
CTACTCG28800.041.499322
GTAGCTG99700.041.3069156
GCTGGGA196700.040.4829989
TACTCGG30050.040.2522543
CCCGAGT37300.040.1563531
TTGGGAG149550.040.1033336
CGGAGGT34900.039.403057
GGAGGCT172050.037.8717849
TAGCTGG114050.037.814077
GCTACTC70050.037.110671
GGGAGGC211600.034.297848
AGGCGGA50750.033.7646944
TCGGGAG38700.033.115476
CTCGGGA36800.032.6731155
GGAGGTT89450.031.6325448
TGGGAGG213900.031.1352847
Produced by FastQC (version 0.11.5)