FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049001
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG34450.082.342993
AGTAGCT129800.079.98535
CCGAGTA36500.078.901782
GAGTAGC84000.077.3124
GTAGCTG139800.073.8003856
TAGCTGG165150.064.521257
GCTGGGA290050.063.9429449
TTGGGAG197900.063.8485346
GCACTTT158900.058.8637431
CCCGAGT50750.058.3134081
ACTTTGG165350.057.6070023
CAAGTAG58800.057.5493163
TTTGGGA183800.057.347595
AAGTAGC62500.057.022314
CCAAGTA60400.056.3824352
AAGTGCT151500.055.3646935
CACTTTG170600.055.0323682
TGAGTAG64400.055.0045783
CTGAGTA65300.054.4669952
CTTTGGG194050.051.4615024
Produced by FastQC (version 0.11.5)