FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162349
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23400.059.3921133
CCGAGTA24050.058.3959272
GAGTAGC57850.054.641584
AGTAGCT93150.054.414955
GTAGCTG96150.051.4441386
GCTGGGA189950.047.6078729
TAGCTGG112100.046.1797647
CCCGAGT32400.045.860861
TTGGGAG131350.044.9481936
CGGAGGT30700.041.7455377
TACTCGG24600.040.6880653
CTACTCG25600.039.6681752
GGAGGCT147350.039.13919
GGGAGGC181350.039.1063358
GCACTTT122000.037.011311
TTTGGGA138100.036.4949535
TCGGGAG32800.036.4386836
GGAGGTT77450.036.255318
CAAGTAG48250.036.1164783
AAGTGCT119850.035.924575
Produced by FastQC (version 0.11.5)