FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084845
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24950.062.0619052
CGAGTAG24700.060.0551953
AGTAGCT92350.057.2241945
GAGTAGC58550.055.8353544
GTAGCTG98700.053.3237576
TACTCGG26300.048.4616053
GCTGGGA194900.048.318839
CTACTCG25950.048.0137832
TAGCTGG114400.047.3273627
CCCGAGT35050.046.921861
TTGGGAG139650.045.781776
GCTACTC63150.041.4630931
GGAGGCT153400.040.692499
TCGGGAG33750.040.318896
CTCGGGA33100.039.588035
GGGAGGC197000.037.0223278
GGAGGTT77150.036.8622368
AAGTGCT118250.036.7753075
TTTGGGA143900.036.624425
CAAGTAG46450.036.120233
Produced by FastQC (version 0.11.5)