FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771121
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT88750.076.174735
CCGAGTA25550.075.250412
CGAGTAG25500.074.537943
GAGTAGC55450.073.619894
GTAGCTG94550.070.8166056
GCTGGGA189450.062.4010969
CCCGAGT32150.062.344741
TAGCTGG113900.060.492557
TTGGGAG137650.056.5932586
CCAAGTA42600.055.1056982
CAAGTAG41750.054.3209953
GCACTTT111500.052.1836741
AAGTAGC45950.051.3927654
GGAGGCT147600.051.266079
TTTGGGA127100.051.037915
ACTTTGG118100.049.6235923
AAGTGCT108000.049.3978585
TCGGGAG32950.047.6337476
CTACTCG28150.047.3240432
CACTTTG121400.047.0966842
Produced by FastQC (version 0.11.5)