FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782149
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26350.080.663313
CCGAGTA26700.078.2566762
AGTAGCT99700.076.618425
GAGTAGC61650.075.111384
GTAGCTG105100.071.517246
TAGCTGG123350.061.753217
GCTGGGA207850.060.9643069
CCCGAGT35950.059.476251
CAAGTAG45000.059.2412033
TTGGGAG142750.057.3452346
AAGTAGC48150.057.28364
CCAAGTA49650.053.9831622
GCACTTT114000.052.719251
TTTGGGA133300.050.9324465
TGAGTAG47750.050.8500253
AAGTGCT109000.049.6051145
CACTTTG123000.049.1463742
ACTTTGG121700.049.138533
GGAGGCT152300.049.116649
CTGAGTA50300.048.8451042
Produced by FastQC (version 0.11.5)