FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782149
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA26650.081.603562
CGAGTAG26150.081.221183
AGTAGCT97600.078.341825
GAGTAGC65950.075.545794
GTAGCTG104900.073.0272756
TTGGGAG146850.064.91326
GCTGGGA213350.063.6455469
TAGCTGG126400.061.972767
GCACTTT118150.061.191591
CCCGAGT37750.061.10231
TTTGGGA134600.058.411265
CAAGTAG42700.057.4970363
ACTTTGG124600.057.0897183
CACTTTG125550.056.0946542
AAGTGCT110100.053.7530945
CCAAGTA45700.053.417412
CTGAGTA52750.053.103912
TGAGTAG52750.052.8211063
CTTTGGG142350.052.6516764
GGAGGCT161300.050.92969
Produced by FastQC (version 0.11.5)